Hematology analyses require EDTA-treated blood (from lavender or purple top tubes containing potassium EDTA only). It is important to ensure that your sample is well mixed and contains optimal proportions of blood and EDTA. The following guidelines should be followed: Fill each tube at least half-full with blood to eliminate dilution errors. Thoroughly mix each sample by gently inverting the filled potassium EDTA sample tube by hand 10 to 15 times to avoid clot formation. If possible, run the sample immediately after mixing. If testing is delayed and the sample is placed on a rocker, be sure to mix it thoroughly again before analysis by gently inverting the tube 10 to 15 times. This ensures the homogeneity of the sample. Do not shake samples. Doing so can damage the blood cells, and can form micro-bubbles that will cause inaccurate results. Never use a rocker for samples smaller than 1.0 ml. Rockers do not mix samples. Feline samples frequently demonstrate platelet aggregations (clumps). Vortex mixing for up to 10 seconds can disaggregate these clumps with no adverse effect on the sample. Some practitioners find that collecting blood from the medial saphenous vein using a vacutainer minimizes platelet clumping.

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For further information on sample collection and handling, refer to our Tech Brief, 'Sample Collection and Handling'.